2019年6月
Label-Free Real-Time Imaging of Extracellular Lactate from a Hippocampal Slice Based on Charge-Transfer-Type Potentiometric Redox Sensor Arrays
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
- 開始ページ
- 617
- 終了ページ
- 620
- 記述言語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.1109/TRANSDUCERS.2019.8808447
© 2019 IEEE. Medically important real-time imaging of extracellular lactate and its spatiotemporal analysis has not yet been achieved. If it could be successfully realized, it would help clarify the function of physiological neurotransmission and drug discovery based on glial cell neuron lactate transport. To this end, we fabricated a 40 μm pitch, 128 × 128 pixel redox-type label-free lactate image sensor based on a potentiometric hydrogen peroxide (H2O2) sensor array and successfully obtained a diffusion image of lactate. Further, the fabricated sensor was applied to extracellular imaging of tissue from a mouse hippocampus. A 300-μm-thick slice of the hippocampus was placed on a lactate image sensor, and when stimulated by glutamate, the output image of the sensor changed dynamically. The output voltage change (ΔVOut) of the sensor was approximately 140 mV. Conversely, verification by a comparative experiment using an enzyme-free sensor confirmed a significant difference in the value of ΔVOut. It can thus be concluded that the observation of lactate in solution was demonstrated successfully by label-free real-time imaging of lactate from mouse hippocampus tissue with glutamate stimulation on a 40 μm-pitch sensor array.
- リンク情報
- ID情報
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- DOI : 10.1109/TRANSDUCERS.2019.8808447
- SCOPUS ID : 85071918273