2001年4月
Quantitative expression studies of aldolase A, B and C genes in developing embryos and adult tissues of Xenopus laevis
MECHANISMS OF DEVELOPMENT
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- 巻
- 102
- 号
- 1-2
- 開始ページ
- 283
- 終了ページ
- 287
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0925-4773(01)00324-0
- 出版者・発行元
- ELSEVIER SCIENCE BV
We previously cloned cDNAs for all the members (A, B and C) of Xenopus aldolase gene family, and using in vitro transcribed RNAs as references, performed quantitative studies of the expression of three aldolase mRNAs in embryos and adult tissues. A Xenopus egg contains ca. 60 pg aldolase A mRNA and ca. 45 pg aldolase C mRNA, but contains only ca. 1.5 pg aldolase B mRNA. The percent composition of three aldolase mRNAs (A:B:C) changes from 56:1.5:42.5 (fertilized egg) to 54:10:36 (gastrula), to 71:14.5:14.5 (neurula) and to 73:20:7 (tadpole) during development. These results are compatible with the previous results of zymogram analysis that aldolases A and C are the major aldolases in early embryos, whose development proceeds depending on yolk as the only energy sourer. Aldolase B mRNA is expressed only late in development in tissues such as pronephros, liver rudiment and proctodeum which are necessary for the future dietary fructose metabolism, and the expression pattern is consistent to that in adult tissues. We also show that three aldolase genes are localized on different chromosomes as single copy genes. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/S0925-4773(01)00324-0
- ISSN : 0925-4773
- Web of Science ID : WOS:000168571500036