MISC

2006年4月

Ex vivo CD4+ T-cell cytokine expression from patients with Sjogren's syndrome following in vitro stimulation to induce proliferation

RHEUMATOLOGY
  • S Koarada
  • ,
  • Y Haruta
  • ,
  • M Mitamura
  • ,
  • F Morito
  • ,
  • Y Tada
  • ,
  • A Ohta
  • ,
  • K Nagasawa

45
4
開始ページ
392
終了ページ
399
記述言語
英語
掲載種別
DOI
10.1093/rheumatology/kei182
出版者・発行元
OXFORD UNIV PRESS

Objective. To assess ex vivo CD4+ T-cell cytokine expression from patients with primary Sjogren's syndrome (SS) following in vitro stimulation to induce proliferation, as proliferation is closely related to differentiation of cytokine-producing cells.
Methods. Peripheral blood mononuclear cells (PBMCs) separated from primary SS patients (n = 28) and controls (n = 25) were analysed. PBMCs were stimulated with concanavalin A followed by phorbol 12-myristate 13-acetate and ionomycin. Intracellular interferon-gamma (IFN-gamma) and interleukin-4 (IL)-4 in proliferating CD4+ T cells were assessed by flow cytometry. The proportion of cytokine-producing cells and proliferating cells in each division cycle was assessed using [5(and 6)-carboxyfluorescein diacetate, succinimidyl ester]-labelled CD4 +/- T cells.
Results. The proportion of IFN-gamma+ proliferating CD4+ T cells in each cell division cycle from extraglandular SS was increased in glandular SS patients compared glandular SS patients with controls (P < 0.05 similar to 0.01). The percentage of IFN-gamma single positive proliferating CD4+ T cells was greater in extraglandular SS patients (26.7 +/- 14.1%) compared with glandular SS (9.9 +/- 9.1%) (P < 0.01) and controls (9.4 +/- 5.8%) (P < 0.001). There was no significant difference in the percentages of IL-4+ proliferating CD4+ T cells among the groups. However, the proliferating response of CD4+ T cells was significantly decreased in extraglandular SS patients (percentage of proliferating cells 38.4 +/- 18.6%) compared with that in glandular SS patients (64.2 +/- 17.2%) (P < 0.05) and controls (63.1 +/- 10.6%) (P < 0.01).
Conclusions. CD4+ T cells from extraglandular SS patients may have a predisposition for entry into the IFN-gamma-producing effector pathway as a result of the stimulations. These results are helpful for understanding the immunological difference between glandular and extraglandular SS and the mechanisms of disease progression.

リンク情報
DOI
https://doi.org/10.1093/rheumatology/kei182
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236252400006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/rheumatology/kei182
  • ISSN : 1462-0324
  • Web of Science ID : WOS:000236252400006

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