2008年1月
Increase of RP105-lacking activated B cells in the peripheral blood and salivary glands in patients with Sjogren's syndrome
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
- 巻
- 26
- 号
- 1
- 開始ページ
- 5
- 終了ページ
- 12
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- CLINICAL & EXPER RHEUMATOLOGY
Objective
To quantify the activated B cells in the peripheral blood and salivary glands of patients with Sjogren's syndrome (SS) by analyzing the expression of RP105 molecule on the B cells.
Methods
The expression of RP105 on the peripheral blood B cells of patients with SS (19 cases) was analyzed by flow cytometry. RP105-positive and negative B cells were sorted and cultured in vitro and the amount of immunoglobulins (IgG and IgM) produced in the supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Salivary gland biopsy samples from 9 SS patients were histologically evaluated and the sequential frozen sections were separately immunostained by anti-RP105 and anti-CD20 monoclonal antibodies.
Results
A significantly higher proportion of peripheral blood RP105-negative B cells was found in SS patients than in healthy individuals. RP105-negative, but not positive, B cells from SS patients were capable of producing IgG and IgM spontaneously in vitro, which was enhanced by the addition of Staphylococcus aureus Cowan I strain (SAC) or IL-6. Salivary glands from 2 of 9 SS patients were found to have lymphoid follicles whose germinal centers consisted of RP105-negative B cells. Moreover, a larger proportion of B cells extensively infiltrating the area other than lymphoid follicles was also RP105-negative.
Conclusion
RP105-negative B cells, a subset of highly activated and well differentiated B cells, which are increased in number in the peripheral blood and extensively infiltrate salivary glands, may be responsible for the production of class-switched immunoglobulin in SS. In. addition, those cells might be associated with the inflammation and tissue damage of the salivary glands.
To quantify the activated B cells in the peripheral blood and salivary glands of patients with Sjogren's syndrome (SS) by analyzing the expression of RP105 molecule on the B cells.
Methods
The expression of RP105 on the peripheral blood B cells of patients with SS (19 cases) was analyzed by flow cytometry. RP105-positive and negative B cells were sorted and cultured in vitro and the amount of immunoglobulins (IgG and IgM) produced in the supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Salivary gland biopsy samples from 9 SS patients were histologically evaluated and the sequential frozen sections were separately immunostained by anti-RP105 and anti-CD20 monoclonal antibodies.
Results
A significantly higher proportion of peripheral blood RP105-negative B cells was found in SS patients than in healthy individuals. RP105-negative, but not positive, B cells from SS patients were capable of producing IgG and IgM spontaneously in vitro, which was enhanced by the addition of Staphylococcus aureus Cowan I strain (SAC) or IL-6. Salivary glands from 2 of 9 SS patients were found to have lymphoid follicles whose germinal centers consisted of RP105-negative B cells. Moreover, a larger proportion of B cells extensively infiltrating the area other than lymphoid follicles was also RP105-negative.
Conclusion
RP105-negative B cells, a subset of highly activated and well differentiated B cells, which are increased in number in the peripheral blood and extensively infiltrate salivary glands, may be responsible for the production of class-switched immunoglobulin in SS. In. addition, those cells might be associated with the inflammation and tissue damage of the salivary glands.
- リンク情報
- ID情報
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- ISSN : 0392-856X
- Web of Science ID : WOS:000253847700002