MISC

2008年6月

Expression of vascular endothelial growth factor-A and mRNA stability factor HuR in human meningiomas

JOURNAL OF NEURO-ONCOLOGY
  • Takahiro Sakuma
  • ,
  • Takao Nakagawa
  • ,
  • Kazunori Ido
  • ,
  • Hiroaki Takeuchi
  • ,
  • Kazufumi Sato
  • ,
  • Toshihiko Kubota

88
2
開始ページ
143
終了ページ
155
記述言語
英語
掲載種別
DOI
10.1007/s11060-008-9559-8
出版者・発行元
SPRINGER

We studied the expression of vascular endothelial growth factor-A (VEGF-A) and mRNA stability factor HuR in 40 supratentorial meningiomas using RT-PCR, ELISA and immunohistochemistry, and analyzed their associations with the clinicopathological characteristics, including microvascular density (MVD), peritumoral brain edema (PTBE), histological subtypes and grades, and the performance of preoperative arterial embolization. Furthermore, we investigated the involvement of HuR in the upregulation of VEGF-A expression using primary meningioma cell cultures. The level of VEGF-A is elevated in meningiomas with PTBE and in higher grade meningiomas. Preoperative arterial embolization did not significantly increase the level of VEGF-A, but it did increase the expression of HuR in tumor tissues. HuR expression was correlated positively with VEGF-A expression in meningioma tissues. In in vitro experiments, hypoxia induced the upregulation of VEGF-A expression and the cytoplasmic translocation of HuR protein in meningioma cells, and inhibition of the cytoplasmic translocation of HuR reduced the upregulation of VEGF-A expression in meningioma cells. These findings suggest that the expression of VEGF-A relates to the development of PTBE with meningiomas and the histological grade, and that HuR is involved in the upregulation of VEGF-A expression in human meningiomas.

リンク情報
DOI
https://doi.org/10.1007/s11060-008-9559-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000256313200003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s11060-008-9559-8
  • ISSN : 0167-594X
  • eISSN : 1573-7373
  • Web of Science ID : WOS:000256313200003

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