2001年6月
Activation of HIV-1-specific immune responses to an HIV-1 vaccine constructed from a replication-defective adenovirus vector using various combinations of immunization protocols.
Clinical and experimental immunology
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- 巻
- 124
- 号
- 3
- 開始ページ
- 445
- 終了ページ
- 52
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1046/j.1365-2249.2001.01540.x
- 出版者・発行元
- BLACKWELL SCIENCE LTD
We constructed a recombinant replication defective adenovirus vector containing the env gene (Ad-Bal) derived from macrophage-trophic HIV-1 (HIV-1 Bal). We then immunized mice with this vector using several administration routes and protocols, and examined the immune response. When the Ad-Bal viral vector (over 1 x 10(7) pfu) was injected subcutaneously, both humoral and cell-mediated immunities were induced. However, immune response induced by the Ad-Bal vector alone was weaker than that induced by the recombinant vaccinia viral vector. We then employed the following three immunization protocols: (l) DNA vaccination followed by immunization with the Ad-Bal; (2) vaccination using the Ad-Bal vector followed by DNA vaccination; and (3) DNA vaccination followed by Ad-Bal infection and passive transfer of dendritic cells (DCs) infected with the Ad-Bal. Among the three protocols, the last gave the strongest humoral and cell-mediated immunity. These results suggest that the combination of DNA vaccination, Ad-Bal vector infection and passive transfer of Ad-Bal-infected DCs can induce strong immunity against HIV-1 Bal.
- リンク情報
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- DOI
- https://doi.org/10.1046/j.1365-2249.2001.01540.x
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11472406
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906078
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000169880900015&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1046/j.1365-2249.2001.01540.x
- ISSN : 0009-9104
- PubMed ID : 11472406
- PubMed Central 記事ID : PMC1906078
- Web of Science ID : WOS:000169880900015