2020年7月10日
Preparation of N2-Ethyl-2′-deoxyguanosine-d4 as an internal standard for the electrospray ionization-tandem mass spectrometric determination of DNA damage by acetaldehyde
Analytical Sciences
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- 巻
- 36
- 号
- 7
- 開始ページ
- 877
- 終了ページ
- 880
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2116/analsci.19N034
The deuteration of N2-ethyl-2'-deoxyguanosine (Et-dG), which is a DNA adduct generated from acetaldehyde, was studied by the addition reaction of acetaldehyde-d4 to 2'-deoxyguanosine (dG) in deuterium oxide (D2O), with the aim to obtain an isotope internal standard for the liquid chromatography/tandem mass spectrometry (LC/MS/MS) quantitation of Et-dG. The replacement of the dG C-8 hydrogen atom by a deuteron atom took place at 50°C in D2O and afforded a mixture of Et-dG-d4 and Et-dG-d5. Et-dG-d4, which was stable in aqueous solutions, was prepared by incubating the mixture in H2O at 60°C for 48 h. The calibration curve was obtained by multiple reaction monitoring (MRM) measurements using a hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometric (HILIC/ESI-MS/MS) system between the Et-dG concentration, ranging from 1.0 × 10-10 to 4.0 × 10-9 M in the sample solutions, and the relative peak areas of Et-dG (m/z: 296.1 → 180.1) to the value of Et-dG-d4 (m/z: 300.2 → 184.2), with an internal standard showing good linearity (R2 = 0.995, n = 5).
- リンク情報
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- DOI
- https://doi.org/10.2116/analsci.19N034
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31983713
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85088103225&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85088103225&origin=inward
- ID情報
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- DOI : 10.2116/analsci.19N034
- ISSN : 0910-6340
- eISSN : 1348-2246
- PubMed ID : 31983713
- SCOPUS ID : 85088103225