論文

国際誌
2018年1月15日

Progress in a selective method for the determination of the acetaldehyde-derived DNA adducts by using HILIC-ESI-MS/MS

Talanta
  • Hiroya Murakami
  • ,
  • Ruri Horiba
  • ,
  • Tomoko Iwata
  • ,
  • Yuta Miki
  • ,
  • Bunji Uno
  • ,
  • Tadao Sakai
  • ,
  • Kazuhiro Kaneko
  • ,
  • Yasushi Ishihama
  • ,
  • Norio Teshima
  • ,
  • Yukihiro Esaka

177
開始ページ
12
終了ページ
17
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.talanta.2017.09.055
出版者・発行元
ELSEVIER SCIENCE BV

Acetaldehyde (AA), which is present in tobacco smoke, automobile exhaust gases and alcohol beverage, is a mutagen and carcinogen. AA reacts with 2'-deoxyguanosine (dG) in DNA to form N2-ethyl-dG (EtdG) and cyclic, 1, N2-propano-dG (CPrdG), which are considered to have a critical role in carcinogenesis induced by AA. In this study, we have developed a highly sensitive method for the quantitation of the two AA-derived DNA adducts by using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) in which hydrophilic interaction chromatography (HILIC) employing mobile phases of high organic solvent concentration was selected to improve the ionization efficiency in the ESI process. Fourteen times and 11 times larger peak areas for EtdG and CPrdG, respectively, in HILIC-ESI-MS/MS were obtained compared with those in reversed phase (RP)-LC-ESI-MS/MS. Furthermore, 6.9 times (for EtdG) and 2.4 times (for CPrdG) larger peak areas were also obtained as additional enhancement by varying additive compounds in the HILIC mobile phases from ammonium acetate to ammonium bicarbonate. In total, the enhancements in detected MS signal intensities by exchanging from the RP-LC system to the HILIC system are 97 times for EtdG and 26 times for CPrdG, respectively. Three commercially available HILIC columns with different polar functional groups were examined and sufficient separation between normal 2'-deoxynucleosides and the AA-derived DNA adducts was achieved by a carbamoyl-bonded HILIC column. Finally, we applied the established method to quantify EtdG and CPrdG in the damaged calf thymus DNA.

リンク情報
DOI
https://doi.org/10.1016/j.talanta.2017.09.055
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702256889445093
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29108566
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000416394300003&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030530201&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85030530201&origin=inward
ID情報
  • DOI : 10.1016/j.talanta.2017.09.055
  • ISSN : 0039-9140
  • eISSN : 1873-3573
  • J-Global ID : 201702256889445093
  • PubMed ID : 29108566
  • SCOPUS ID : 85030530201
  • Web of Science ID : WOS:000416394300003

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