論文

国際誌
2019年9月1日

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Electrophoresis
  • Júlia Lačná
  • ,
  • Jan Přikryl
  • ,
  • Norio Teshima
  • ,
  • Hiroya Murakami
  • ,
  • Yukihiro Esaka
  • ,
  • František Foret
  • ,
  • Petr Kubáň

40
18-19
開始ページ
2390
終了ページ
2397
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/elps.201900112

In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4 D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.

リンク情報
DOI
https://doi.org/10.1002/elps.201900112
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31218732
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068158096&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85068158096&origin=inward
ID情報
  • DOI : 10.1002/elps.201900112
  • ISSN : 0173-0835
  • eISSN : 1522-2683
  • PubMed ID : 31218732
  • SCOPUS ID : 85068158096

エクスポート
BibTeX RIS