2013年7月
Identification of a Hepatoprotective Peptide in Wheat Gluten Hydrolysate against D-Galactosamine-Induced Acute Hepatitis in Rats
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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回数 : 219
- 巻
- 61
- 号
- 26
- 開始ページ
- 6304
- 終了ページ
- 6310
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jf400914e
- 出版者・発行元
- AMER CHEMICAL SOC
A hepatoprotective peptide, pyroglutamyl leucine (pyroGlu-Leu), was identified in wheat gluten hydrolysate through an in vivo activity-guided fractionation approach based on D-galactosamine-induced acute hepatitis in rats and fractionation of peptides with large-scale preparative ampholine-free isoelectric focusing. The active acidic fraction predominantly consisted of pyroglutamyl peptides and free pyroglutamic acid. Pyroglutamyl peptides were derivatized with phenyl isothiocyanate after removal of a pyroglutamyl residue by pyroglutamate aminopeptidase. The derivatives were purified by reversed-phase HPLC and subjected to sequence analysis. The active fraction contained pyroGlu-Ile, pyroGlu-Leu, pyroGlu-Gln, pyroGlu-Gln-Gln, and free pyroGlu. Ingestion of pyroGlu-Leu at 20 mg/kg body weight significantly decreased serum aspartate and alanine aminotransferases to approximately 30% and 20% of those values of the vehicle group, respectively, which were near the normal levels. Thirty minutes after ingestion of pyroGlu-Leu at 20 mg/kg, the concentration of pyroGlu-Leu in portal blood plasma increased to approximately 2 mu M.
- リンク情報
- ID情報
-
- DOI : 10.1021/jf400914e
- ISSN : 0021-8561
- Web of Science ID : WOS:000321542800022