2006年7月
Improvement in isolation and identification of food-derived peptides in human plasma based on precolumn derivatization of peptides with phenyl isothiocyanate
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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プレプリント・著者最終稿
回数 : 125
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- 巻
- 54
- 号
- 15
- 開始ページ
- 5261
- 終了ページ
- 5266
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jf060531s
- 出版者・発行元
- AMER CHEMICAL SOC
For the isolation and detection of food-derived peptides in blood, an approach based on the derivatization of peptides with phenyl isothiocyanate (PITC) was developed. This approach allows hydrophilic peptides to be resolved and specifically detected by reversed-phase (RP) HPLC. For the rapid capturing and clarification of peptides in human plasma, solid-phase extraction by using a mini spin column (5 mm x 5 mm) packed with a strong cation exchanger was used. The clarified peptide fraction was further fractionated by size-exclusion chromatography (SEC). The peptides in the SEC fractions were derivatized with PITC, and the derivatives were resolved by RP-HPLC by using an ammonium acetate buffer or a trifluoroacetic acid system. An automatic peptide sequencer based on Edman degradation with a modified program can directly analyze the resolved derivatives. Some synthetic peptides and food-derived peptides in human plasma were successfully isolated and identified by this approach.
- リンク情報
- ID情報
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- DOI : 10.1021/jf060531s
- ISSN : 0021-8561
- Web of Science ID : WOS:000239120200009