1999年8月
Quantitative analysis of pyroglutamic acid in peptides
Journal of Agricultural and Food Chemistry
- ,
- ,
- 巻
- 47
- 号
- 8
- 開始ページ
- 3248
- 終了ページ
- 3251
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jf990003z
A simplified and rapid procedure for the determination of pyroglutamic acid in peptides was developed. The method involves the enzymatic cleavage of an N-terminal pyroglutamate residue using a thermostable pyroglutamate aminopeptidase and isocratic HPLC separation of the resulting enzymatic hydrolysate using a column switching technique. Pyroglutamate aminopeptidase from a thermophilic archaebacteria, Pyrococcus furiosus, cleaves N-terminal pyroglutamic acid residue independent of the molecular weight of the substrate. It cleaves more than 85% of pyroglutamate from peptides whose molecular weight ranges from 362.4 to 4599.4 Da. Thus, a new method is presented that quantitatively estimates N-terminal pyroglutamic acid residue in peptides.
- ID情報
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- DOI : 10.1021/jf990003z
- ISSN : 0021-8561
- PubMed ID : 10552639
- SCOPUS ID : 0032830678