MISC

2001年7月

Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by fungi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Y Saratani
  • ,
  • E Uheda
  • ,
  • H Yamamoto
  • ,
  • A Nishimura
  • ,
  • F Yoshizako

65
7
開始ページ
1676
終了ページ
1679
記述言語
英語
掲載種別
DOI
10.1271/bbb.65.1676
出版者・発行元
TAYLOR & FRANCIS LTD

The enantioselectivity of ECAA to ECHB by eight fungi of four genus was evaluated. All strains showed (S)-selectivity, and Cylindrocarpon sclerotigenum IFO 31855 gave the highest yield and good optical purity (e.e.; >99%). Cell-free extract and acetone-dried cells of C. sclerotigenum IFO 31855 reduced ECAA to (S)ECHB in the presence of NADPH (e.e.;>99%) and the e.e. was not decreased by heat treatment of the cell-free extract or the acetone-dried cells. The active fractions shown by two peaks on a DEAE-Toyopearl 650 M column gave preferentially (S)-ECHB (e.e.; > 99%).

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1271/bbb.65.1676
CiNii Articles
http://ci.nii.ac.jp/naid/110002693396
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/11515558
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000170238400034&DestApp=WOS_CPL

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