2021年
Detection Time Distribution of Microcolonies Formed by Individual Heat-Injured Cells of Escherichia coli.
Biocontrol science
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- 巻
- 26
- 号
- 4
- 開始ページ
- 211
- 終了ページ
- 215
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.4265/bio.26.211
The microcolony formation at 30℃ on an enriched minimal salts agar plates by individual Escherichia coli cells heated at 50℃ was monitored with a time-lapse shadow image analysis system, MicroBio μ3DTM AutoScanner. While the time course of microcolony count detected every half an hour for the unheated cells seemingly demonstrated a normal distribution, that for the heated cell population demonstrated totally the growth delay probably resulting from cell injury and also interestingly distributed in its rather deformed pattern with a tailing. Those patterns of the cumulative counts of appearing microcolonies during the post-heating cultivation period were expressed in three different mathematical models. This approach may be proposed as a rapid cultivation method predictable for enumeration of viable and repairable injured cells in practical use.
- ID情報
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- DOI : 10.4265/bio.26.211
- PubMed ID : 35013018