論文

査読有り
2012年3月

Interaction of modified tail-anchored proteins with liposomes: effect of extensions of hydrophilic segment at the COOH-terminus of holo-cytochromes b₅.

Journal of bioscience and bioengineering
  • Yoichi Sakamoto
  • ,
  • Masahiro Miura
  • ,
  • Fusako Takeuchi
  • ,
  • Sam-Yong Park
  • ,
  • Motonari Tsubaki

113
3
開始ページ
322
終了ページ
31
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2011.11.004
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

A group of membrane proteins having a single COOH-terminal hydrophobic domain capable of post-translational insertion into lipid bilayer is known as tail-anchored (TA) proteins. To clarify the insertion mechanism of the TA-domain of human cytochrome b(5) (Hcytb5) into ER membranes, we produced and purified various membrane-bound forms of Hcytb5 with their heme b-bound, in which various truncated forms of NH(2)-terminal bovine opsin sequence were appended at the COOH-terminus of the native form. We analyzed the integration of the TA-domains of these forms onto protein-free liposomes. The integration occurred efficiently even in the presence of a small amount of sodium cholate and, once incorporated, such proteoliposomes were very stable. The mode of the integration was further analyzed by treatment of the proteoliposomes with trypsin either on the extravesicular side or on the luminal side. LC-MS analyses of the trypsin digests obtained from the proteoliposomes indicated that most of the C-terminal hydrophilic segment of the native Hcytb5 were exposed towards the lumen of the vesicles and, further, a significant part of the population of the extended C-terminal hydrophilic segments of the modified Hcytb5 were exposed in the lumen as well, suggesting efficient translocation ability of the TA-domain without any assistance from other protein factors. Present results opened a route for the use of the C-terminal TA-domain as a convenient tool for the transport of proteins as well as short peptides into artificial liposomes.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2011.11.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22138382
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000302505700008&DestApp=WOS_CPL