渡部博之 (Hiroyuki Watabe) - マイポータル - researchmap

基本情報

所属: 北海道医療大学薬学部薬学科生化学講座教授

学位: 医学博士(北海道大学)

J-GLOBAL ID: 200901016941384082
researchmap会員ID: 1000060336

研究キーワード

6

研究分野

4

経歴

8

1978年 - 1981年

北海道医療大学(旧東日本学園大学)薬学部助教授
1978年 - 1981年

Health Sciences Univ. of Hokkaido, Associate professor
1981年

- 北海道医療大学(旧東日本学園大学)薬学部教授
1981年

- Health Sciences Univ. of Hokkaido, Professor
1976年 - 1978年

北海道医療大学(旧東日本学園大学)薬学部講師
1976年 - 1978年

Health Sciences Univ. of Hokkaido, Faculty of Pharm. Sci.
1966年 - 1976年

北海道大学医学部助手
1966年 - 1976年

Hokkaido Univ. School of Medicine・Instructor

学歴

2

- 1964年

北海道大学, 医学部, 薬学科
- 1964年

北海道大学

委員歴

1

1988年

日本生化学会, 評議員

MISC

23

A new method for detecting single nucleotide polymorphism using GFP-display

T Aoki, K Satoh, T Imamura, H Watabe

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 60(1) 61-67 2004年7月
General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides

T Aoki, T Tahara, K Satoh, H Fujino, H Watabe

ANALYTICAL BIOCHEMISTRY 317(1) 107-115 2003年6月
A highly sensitive assay for proteases using staphylococcal protein A fused with enhanced green fluorescent protein

H Fujino, T Aoki, H Watabe

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 66(7) 1601-1604 2002年7月
Application of green fluorescent protein to affinity electrophoresis; affinity of IgG-binding domain C from streptococcal protein G to mouse IgG1.

Hitoshi Kazama, Keiko Yamada, Takashi Aoki, Hiroyuki Watabe

Biol. Pharm. Bull. 25(2) 168-171 2002年
"GFP-display," an easy detection method for single amino acid changes in a target polypeptide: Application to random mutagenesis

T Aoki, T Tahara, H Fujino, H Watabe

ANALYTICAL BIOCHEMISTRY 300(1) 103-106 2002年1月
A flexible single-step detection of blotted antigen using a fusion protein between protein A and green fluorescent protein

T Aoki, M Miyashita, H Fujino, H Watabe

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 64(7) 1547-1551 2000年7月
A new approach to gene mutation analysis using "GFP-display"

T Aoki, R Ami, H Onagi, H Fujino, H Watabe

JOURNAL OF BIOCHEMISTRY 127(4) 627-633 2000年4月
Application of Green Fluorescent Protein-Protein A Fusion Protein to Western Blotting

Methods Enzymol. 302 264-272 1999年
A Milk Cloffing Assay for Antibody Using a Fusion Protein between Protein A and Pepsinogen C

Biol. Pharm. Bull. 22(3) 317-319 1999年
Detection of blotted antigen using a fusion protein between protein A and pepsinogen C

T Aoki, M Satoh, H Watabe

JOURNAL OF BIOCHEMISTRY 124(3) 572-577 1998年9月
Purification of recombinant human pepsinogens and their application as immunoassay standards

T Aoki, E Tomaki, M Satoh, M Takashiro, H Onagi, M Itoh, T Teramoto, J Morikawa, H Watabe

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 45(2) 289-301 1998年6月
Biochemical and Immunochemical Properties of Modified Human Neuron-Specific Enolase

Mitsuhiro KANETA, Takashi AOKI, Hitoshi ONAGI, Junji MORIKAWA, Hiroyuki WATABE

Biol. Pharm. Bull. 20(5) 556-559 1997年
Purification of pepsinogens from human urine and electrophoretic analysis by caseogram print

T Aoki, E Tomaki, J Morikawa, N Tsubota, H Watabe

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 39(6) 1147-1156 1996年8月
Modification of human neuron-specific enolase for application to radioimmunoassay

T Aoki, M Kaneta, H Onagi, J Morikawa, N Tsubota, H Watabe

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 39(3) 461-469 1996年6月
Construction of a fusion protein between protein A and green fluorescsent protein and its application to Western blotting

FEBS Lett. 384,193-197 1996年
Conversion of cathepsin E to enggmatic unstable from in gastric cancer cells

Biol. Pharm. Bull. 18,1522-1525 1995年
ELECTROPHORETIC ANALYSIS OF A GASTRIC CANCER-ASSOCIATED ACID PROTEINASE USING A HIGHLY SENSITIVE DETECTION SYSTEM

T AOKI, T TAKASAKI, J MORIKAWA, T YANO, H WATABE

BIOLOGICAL & PHARMACEUTICAL BULLETIN 17(10) 1358-1363 1994年10月
Appearance of AFP during Ontogenesis and Oncogenesis in Rats

Journal of Tumor Marker Oncology 9(1) 91-98 1994年
CHARACTERIZATION OF RECOMBINANT HUMAN NEURON-SPECIFIC ENOLASE AND ITS APPLICATION TO ENZYME-IMMUNOASSAY

T AOKI, M KIMURA, M KANETA, H KAZAMA, J MORIKAWA, H WATABE

TUMOR BIOLOGY 14(5) 261-270 1993年9月
DETECTION OF TETRACYCLINE RESISTANCE PROTEIN ENCODED BY BACILLUS-SUBTILIS PLASMID PNS1

T AOKI, M KANETA, H WATABE

BIOLOGICAL & PHARMACEUTICAL BULLETIN 16(1) 81-83 1993年1月

もっとみる

所属学協会

4

共同研究・競争的資金等の研究課題

6