1998年10月
Asymmetrical membrane fluidity of bovine adrenal chromaffin cells and granules and effect of trichosporin-B-VIa
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
- ,
- ,
- ,
- ,
- ,
- 巻
- 1375
- 号
- 1-2
- 開始ページ
- 93
- 終了ページ
- 100
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0005-2736(98)00143-6
- 出版者・発行元
- ELSEVIER SCIENCE BV
We examined membrane fluidity of bovine adrenal chromaffin cells and chromaffin granules using cationic trimethylammonium derivative of diphenylhexatriene (TMA-DPH) as a fluorescence probe. After adding TMA-DPH to the suspension of chromaffin cells and that of granules, it first bound to the outer layer of the plasma membrane of the dells and that of the granule membrane, then gradually penetrated the inner layer of each membrane and distributed to both leaflets of the respective membranes. Accompanying increases in the ratio of incorporated probe on the cytoplasmic side of the chromaffin cell membrane, its fluorescence anisotropy gradually decreased. However, in chromaffin granules,the fluorescence anisotropy gradually increased with increases in the ratio of incorporated probe. These findings suggest that the inner layer of the plasma membrane and outer layer of the granular membrane are more fluid than the corresponding side of each membrane, which is suitable for the fusion between both membranes. We also examined the effect of trichosporin-B VIa, a fungal ion channel forming alpha-aminoisobutyric acid-containing peptide, on the fluidity of chromaffin cells using TMA-DPH. The peptide decreased the fluorescence anisotropy and increased the fluorescence intensity in the concentration range that induced Ca2+ dependent catecholamine secretion, suggesting that a change in lipid dynamics of the lipid bilayer of the plasma membrane was induced by this peptide. (C) 1998 Elsevier Science B.V. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/S0005-2736(98)00143-6
- ISSN : 0005-2736
- Web of Science ID : WOS:000076520000011