Thirty-two strains of white rot fungi were cultivated on empty fruit bunch (EFB) of oil palm (Elaeis guineensis) to find potential candidates for bio-delignification of EFB. To produce ethanol. EFB was cultured with the selected fungal strains, and then subjected to ethanolysis. Ethanol was produced from the pulp fraction by simultaneous saccharification and fermentation (SSF). Several white rot fungi including Pleurotus ostreatus. Ceriporiopsis subvermispora and Dichomitus squalens promoted production of ethanol from EFB by SSF.