1996年11月
Reaction of chemically modified lignin peroxidase of Phanerochaete chrysosporium in water-miscible organic solvents
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- ,
- 巻
- 60
- 号
- 11
- 開始ページ
- 1805
- 終了ページ
- 1809
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.60.1805
- 出版者・発行元
- TAYLOR & FRANCIS LTD
LiP of Phanerochaete chrysosporium was chemically modified with MPSS, and acetic and benzoic acid N-hydroxysuccinimide esters to increase the activity in organic solvents. IEF demonstrated the LiP was chemically modified with these modifers. The modification of LiP was also confirmed by GFC and anion-exchange chromatography analyses. In addition, the modified LiP retained its original activity in aqueous solution. Furthermore, these modified LiPs had higher 3,3'-dimethoxybenzidine oxidation activity than the native LiP in aqueous 70% water-miscible organic solutions including ethylene glycol and diethylene glycol. However, the activities of these modified enzymes were found to be negligibly low in water-immiscible organic solvents such as benzene and toluene. Furthermore, the effects of these introducing groups and the properties of organic solvents on the reaction of LiP in water-miscible organic solvents were investigated. The reaction of LiP modified with aliphatic acid was found to depend on the viscosity of the reaction system containing 70% water-miscible organic solvents. This suggests that the viscosity of the reaction system containing water-miscible organic solvents is important for the reaction of LiP modified with aliphatic acids.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.60.1805
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902151338194498
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002678375
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VV25200012&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.60.1805
- ISSN : 0916-8451
- eISSN : 1347-6947
- J-Global ID : 200902151338194498
- CiNii Articles ID : 110002678375
- Web of Science ID : WOS:A1996VV25200012