論文

2004年5月

Characterization of Kdap, a protein secreted by keratinocytes

JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • S Tsuchida
  • ,
  • M Bonkobara
  • ,
  • McMillan, JR
  • ,
  • M Akiyama
  • ,
  • T Yudate
  • ,
  • Y Aragane
  • ,
  • T Tezuka
  • ,
  • H Shimizu
  • ,
  • PD Cruz
  • ,
  • K Ariizumi

122
5
開始ページ
1225
終了ページ
1234
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.0022-202X.2004.22511.x
出版者・発行元
NATURE PUBLISHING GROUP

Using a signal sequence-trap we identified a human gene encoding a polypeptide of 99 amino acids with a putative signal sequence. The gene was identical to keratinocyte differentiation-associated protein (Kdap), which was reported previously by Oomizu et al (Gene 256: 19-27, 2000) to be expressed in embryonal rat epidermis at the mRNA level. In humans, we found Kdap mRNA expression to be restricted to epithelial tissue at high levels. The 12.5 kDa protein was detected in culture supernatant of keratinocytes and those transfected adenovirally with the Kdap gene. In normal skin, Kdap protein was found exclusively within lamellar granules of granular keratinocytes and in the intercellular space of the stratum corneum. By contrast, in lesional skin of patients with psoriasis, Kdap was expressed more widely throughout suprabasal keratinocytes. When induced to differentiate in vitro, keratinocytes showed marked upregulation of Kdap mRNA expression similar to that of involucrin mRNA, but with differing kinetics. Finally, a spliced variant of Kdap mRNA was generated by alternative splicing mechanisms. Our studies indicate that human Kdap resembles rat Kdap with respect to tissue and cell expression at the mRNA level and that Kdap is a low-molecular-weight protein secreted by keratinocytes. Thus Kdap may serve as a soluble regulator of keratinocyte differentiation.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1111/j.0022-202X.2004.22511.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221301000028&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.0022-202X.2004.22511.x
  • ISSN : 0022-202X
  • Web of Science ID : WOS:000221301000028

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