論文

国際誌
2020年5月22日

Molecular sexing in Japanese murrelet (Synthliboramphus wumizusume) and a tandem-repeat polymorphism on the W chromosome.

Scientific reports
  • Hitoshi Hatakeyama
  • ,
  • Yutaka Nakamura
  • ,
  • Takahiro Konaka
  • ,
  • Shin Nishida
  • ,
  • Wannapimol Kriangwanich
  • ,
  • Kazuyoshi Uematsu
  • ,
  • Shuichi Tsuchida

10
1
開始ページ
8576
終了ページ
8576
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-65206-7

The Japanese murrelet (Synthliboramphus wumizusume) is an endangered small seabird species in Japan. Molecular sexing using PCR targeting of the gene encoding chromodomain helicase DNA-binding protein 1(CHD1) has been used for sex identification. Specifically, PCR using any of three commonly used primer sets (CHD1F/1R, 2550F/2718R and P2/P8) has permitted sexing in many bird species. CHD1F/1R and 2550F/2718R permitted molecular sexing in Japanese murrelet; however, P2/P8 did not permit. To generate a primer pair that permits efficient molecular sexing in this species, a new primer set, CHD1F1/1R1, was prepared to permit amplification of smaller products from degraded DNA samples. The electrophoretic patterns of PCR products amplified with the new primer set were easily classified as female or male. Additionally, the PCR product indicated the presence of a polymorphism in the fragment from chromosome W. The PCR fragments of long-type (WL) and short-type (WS) polymorphisms were observed only in females. When the distribution of the CHD1 gene on chromosome W of 61 female Japanese murrelet on Biroujima Island in Miyazaki Prefecture, WL and WS were observed in 90.2% and 9.8%. The DNA polymorphism is derived from the number of copies of a 32-bp-repeat unit, with WL and WS corresponding to two and one 32-bp-repeats, respectively.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-65206-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32444700
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244538
ID情報
  • DOI : 10.1038/s41598-020-65206-7
  • PubMed ID : 32444700
  • PubMed Central 記事ID : PMC7244538

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