論文

査読有り
2007年4月

In vivo visualization and portally repeated transplantation of bone marrow cells in rats with liver damage

STEM CELLS AND DEVELOPMENT
  • Junko Haga
  • ,
  • Go Wakabayashi
  • ,
  • Motohide Shimazu
  • ,
  • Minoru Tanabe
  • ,
  • Takeshi Takahara
  • ,
  • Toshifumi Azuma
  • ,
  • Yuki Sato
  • ,
  • Yoji Hakamata
  • ,
  • Eiji Kobayashi
  • ,
  • Masaki Kitajima

16
2
開始ページ
319
終了ページ
327
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/scd.2006.0086
出版者・発行元
MARY ANN LIEBERT INC

Recent reports have raised concerns over the feasibility of differentiating bone marrow cells (BMCs) into functional hepatocytes. Such augmentation is considered necessary for potential clinical use of these cells in liver diseases. The present investigation was designed to determine the kinetics of transplanted BMCs and evaluate the effects of repeated bone marrow transplantation (BMT) in rat models of CCl4-induced liver damage. The early kinetics of transplanted BMCs was evaluated with a charge-coupled-device (CCD) camera using BMCs obtained from green fluorescent protein (GFP) transgenic (Tg) rats and followed up with in vivo imaging system (IVIS) using BMCs obtained from firefly luciferase (luc) Tg rats. We used a portal infusion system for repeated BMT. BMCs were transplanted via a peripheral vein or the portal vein (PV) once or repeatedly using this system. The results revealed that BMCs accumulated more in the damaged liver than in the intact liver. In the experimental group receiving repeated BMT via the PV, the liver fibrosis was milder than that in the group not receiving BMT, and large clusters of albumin-producing cells were detected by albumin staining. The injected BMCs were shown to accumulate in the damaged liver. This strategy of repeated BMT has potential clinical use in enhancing the number of albumin-producing cells and suppressing liver fibrosis. This combination of beneficial effects may contribute to the benefits of cell transplantation therapy. Demonstration of the benefits of BMT in this study may be expected to have great significance for clinical trials.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1089/scd.2006.0086
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17521242
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000246713800012&DestApp=WOS_CPL