論文

査読有り
2007年3月

Oligodendrocytes and radial glia derived from adult rat spinal cord progenitors: Morphological and immunocytochemical characterization

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
  • Iris Kulbatski
  • ,
  • Andrea J. Mothe
  • ,
  • Armand Keating
  • ,
  • Yoji Hakamata
  • ,
  • Eiji Kobayashi
  • ,
  • Charles H. Tator

55
3
開始ページ
209
終了ページ
222
記述言語
英語
掲載種別
DOI
10.1369/jhc.6A7020.2006
出版者・発行元
HISTOCHEMICAL SOC INC

Self-renewing, multipotent neural progenitor cells (NPCs) reside in the adult mammalian spinal cord ependymal region. The current study characterized, in vitro, the native differentiation potential of spinal cord NPCs isolated from adult enhanced green fluorescence protein rats. Neurospheres were differentiated, immunocytochemistry (ICC) was performed, and the positive cells were counted as a percentage of Hoescht+ nuclei in 10 random fields. Oligodendrocytes constituted most of the NPC progeny (58.0% of differentiated cells; 23.4% in undifferentiated spheres). ICC and electron microscopy (EM) showed intense myelin production by neurospheres and progeny. The number of differentiated astrocytes was 18.0%, but only 2.8% in undifferentiated spheres. The number of differentiated neurons was 7.4%, but only 0.85% in undifferentiated spheres. The number of differentiated radial glia (RG) was 73.0% and in undifferentiated spheres 80.9%. EM showed an in vitro phagocytic capability of NPCs. The number of undifferentiated NPCs was 32.8% under differentiation conditions and 78.9% in undifferentiated spheres. Compared with ependymal region spheres, the spheres derived from the peripheral white matter of the spinal cord produced glial-restricted precursors. These findings indicate that adult rat spinal cord ependymal NPCs differentiate preferentially into oligodendrocytes and RG, which may support axonal regeneration in future trials of transplant therapy for spinal cord injury.

Web of Science ® 被引用回数 : 54

リンク情報
DOI
https://doi.org/10.1369/jhc.6A7020.2006
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17101728
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000244375700002&DestApp=WOS_CPL