The morphology of macropores in the root zones of grassland in Brown forest soil, England, was investigated in the present study. The structure of macropores was determined by three dimensional stereoscopic-radiography of macropore morphology and examination of the physical condition of the soil. The soil samples were collected from four layers of root-zones that were shallower than 85cm in depth in a 14-year-old perennial ryegrass pasture. Each soil layer had a clay texture, and was dense and solid. The porosity rate was between 54% (surface layer; 0-5cm) and 38% (subsoil layer; 80-85cm). The non-capillary pores of the first soil layer (0-10cm) consisted of pores formed by earthworms and aggregates, and in the second to the fourth soil layers (10-85cm) the pores were formed by roots. The capillary pores in the surface layer (first layer) consisted of pores formed by roots and pores of inter-particles and inter-aggregates. In the subsoil layer, the capillary pores were formed by roots. Thus, similar to the pore morphology of the Andosols, Steppe soil cases and Red yellow soil, most macropores of grassland in Brown forest soil, England, are tubular in shape and formed by plant roots.