Papers

Peer-reviewed
May, 2014

Temporal Changes in Prosaposin Expression in the Rat Dentate Gyrus after Birth

PLOS ONE
  • Midori Morishita
  • ,
  • Hiroaki Nabeka
  • ,
  • Tetsuya Shimokawa
  • ,
  • Kyojy Miyawaki
  • ,
  • Takuya Doihara
  • ,
  • Shouichiro Saito
  • ,
  • Naoto Kobayashi
  • ,
  • Seiji Matsuda

Volume
9
Number
5
First page
e95883
Last page
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1371/journal.pone.0095883
Publisher
PUBLIC LIBRARY SCIENCE

Neurogenesis in the hippocampal dentate gyrus occurs constitutively throughout postnatal life. Adult neurogenesis includes a multistep process that ends with the formation of a postmitotic and functionally integrated new neuron. During adult neurogenesis, various markers are expressed, including GFAP, nestin, Pax6, polysialic acid-neural cell adhesion molecule (PSA-NCAM), neuronal nuclei (NeuN), doublecortin, TUC-4, Tuj-1, and calretinin. Prosaposin is the precursor of saposins A-D; it is found in various organs and can be excreted. Strong prosaposin expression has been demonstrated in the developing brain including the hippocampus, and its neurotrophic activity has been proposed. This study investigated changes in prosaposin in the dentate gyrus of young and adult rats using double immunohistochemistry with antibodies to prosaposin, PSA-NCAM, and NeuN. Prosaposin immunoreactivity was intense in the dentate gyrus at postnatal day 3 (P3) and P7, but decreased gradually after P14. In the dentate gyrus at P28, immature PSA-NCAM-positive neurons localized exclusively in the subgranular zone were prosaposin-negative, whereas mature Neu-N-positive neurons were positive for prosaposin. Furthermore, these prosaposin-negative immature neurons were saposin B-positive, suggesting that the neurons take up and degrade prosaposin. In situ hybridization assays showed that prosaposin in the adult dentate gyrus is dominantly the Pro+9 type, a secreted type of prosaposin. These results imply that prosaposin secreted from mature neurons stimulates proliferation and maturation of immature neurons in the dentate gyrus.

Link information
DOI
https://doi.org/10.1371/journal.pone.0095883
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24871372
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336858300003&DestApp=WOS_CPL
ID information
  • DOI : 10.1371/journal.pone.0095883
  • ISSN : 1932-6203
  • Pubmed ID : 24871372
  • Web of Science ID : WOS:000336858300003

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