2014年4月
PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation
AUTOPHAGY
- 巻
- 10
- 号
- 4
- 開始ページ
- 631
- 終了ページ
- 641
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.4161/auto.27785
- 出版者・発行元
- LANDES BIOSCIENCE
Skeletal muscle atrophy is thought to result from hyperactivation of intracellular protein degradation pathways, including autophagy and the ubiquitin-proteasome system. However, the precise contributions of these pathways to muscle atrophy are unclear. Here, we show that an autophagy deficiency in denervated slow-twitch soleus muscles delayed skeletal muscle atrophy, reduced mitochondrial activity, and induced oxidative stress and accumulation of PARK2/Parkin, which participates in mitochondrial quality control (PARK2-mediated mitophagy), in mitochondria. Soleus muscles from denervated Park2 knockout mice also showed resistance to denervation, reduced mitochondrial activities, and increased oxidative stress. In both autophagy-deficient and Park2-deficient soleus muscles, denervation caused the accumulation of polyubiquitinated proteins. Denervation induced proteasomal activation via NFE2L1 nuclear translocation in control mice, whereas it had little effect in autophagy-deficient and Park2-deficient mice. These results suggest that PARK2-mediated mitophagy plays an essential role in the activation of proteasomes during denervation atrophy in slow-twitch muscles.
- リンク情報
-
- DOI
- https://doi.org/10.4161/auto.27785
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24451648
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335547800008&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898478184&doi=10.4161%2fauto.27785&partnerID=40&md5=80c0acc222a0b4c76b75b56212f0cf39
- ID情報
-
- DOI : 10.4161/auto.27785
- ISSN : 1554-8627
- eISSN : 1554-8635
- PubMed ID : 24451648
- Web of Science ID : WOS:000335547800008