2003年5月
Prostaglandin E-2 induced the differentiation of osteoclasts in mouse osteoblast-depleted bone marrow cells
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
- ,
- ,
- ,
- ,
- ,
- 巻
- 68
- 号
- 5
- 開始ページ
- 351
- 終了ページ
- 358
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0952-3278(03)00027-9
- 出版者・発行元
- CHURCHILL LIVINGSTONE
Prostaglandin (PG) E-2 is a known bone absorbing agent that acts on osteoblasts to facilitate osteoclastogenesis by increasing the secretion of RANKL. In the present study, we investigated the direct action of PGE(2) on osteoclastic progenitors that differentiate into TRAP-positive multinucleated cells. The hematopoietic stem cell obtained from murine bone marrow was purified by a Sephadex G-10 column, and cultured in the presence of CSF-1 and RANKL to facilitate cell differentiation. The introduction of low-density PGE2 into the culture resulted in a drastic increase of TRAP-positive multinucleated cells, whereas the addition of high-density PGE2 had the opposite effect. PCR analysis revealed increased level of EP3 mRNA in undifferentiated cells and reduced level after the development of osteoclast; EP1, EP2 and EP4 were constitutively expressed throughout the differentiation. Investigation of intracellular signaling verified that low-density PGE2 suppressed PKA activity in undifferentiated cells, suggesting that PGE2 acts on the osteoclastic cell lineage to facilitate cell differentiation by suppressing PKA in the presence of RANKL. (C) 2003 Elsevier Science Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/S0952-3278(03)00027-9
- ISSN : 0952-3278
- Web of Science ID : WOS:000182762800009