Many studies have focused on the effects of tourism on mental health. This study revealed that short-stay vacations at mental health rejuvenation facilities could reduce Japanese employees' stress levels. We conducted two studies with two groups of participants (N=34 and 26, respectively), in order to obtain both objective and subjective evaluations. The results suggested that taking a short-stay vacation at a mental health rejuvenation facility was effective for helping employees to maintain good mental health, and that stress reduction was particularly evident in the highly stressed group. Practical and theoretical implications are discussed with a view to providing suggestions for addressing work-related stress in Japanese employees.