Tadashi Asakura - My portal - researchmap

Profile Information

Affiliation: Lecturer, Faculty of Medicine, School of Medicine, Department of Biochemistry Ⅰ, Jikei University

Degree: (BLANK)

J-GLOBAL ID: 200901098077112209
researchmap Member ID: 1000078454

Research Interests

4

Research Areas

1

Life sciences / Cell biology /

Research History

7

Apr, 2016 - Present

Professor, Radioisotope Research Faculties, Jikei University School of Medicine
Apr, 2008 - Mar, 2016

Associate Professor, Biochemistry, Jikei University School of Medicine
Apr, 1995 - Mar, 2008

Assistant Professor, Biochemistry, Jikei University School of Medicine
1980 - 1995

Jikei University
1980 - 1995

Research Associate, Jikei University School of
1995

- Assistant Professor, Jikei University School of
Medicine

Education

2

- 1980

Faculty of Science, Tokyo Metropolitan University
- 1980

Faculty of Science, Tokyo Metropolitan University

Papers

3

Chemotherapeutic Effect of Anti-CD147 Antibody Labelled Micelles Encapsulating a Conjugate of Doxorubicin with Glutathione Targeting CD147-Expressing Carcinoma Cells.

ASAKURA Tadashi

Anticancer Res, 38 1311-1316, 2018 Peer-reviewed
Antibody to human alpha-fetoprotein inhibits cell growth of human hepatocellular carcinoma cells by resuscitating the PTEN molecule: in vitro experiments

Kiyoshi Ohkawa, Tadashi Asakura, Yutaka Tsukada, Tomokazu Matsuura

INTERNATIONAL JOURNAL OF ONCOLOGY, 50(6) 2180-2190, Jun, 2017 Peer-reviewed
Proteasome inhibitor-resistant cells cause EMT-induction via suppression of E-cadherin by miR-200 and ZEB1

Tadashi Asakura, Noriko Yamaguchi, Kiyoshi Ohkawa, Kiyotsugu Yoshida

INTERNATIONAL JOURNAL OF ONCOLOGY, 46(5) 2251-2260, May, 2015 Peer-reviewed

Misc.

44

Three-dimensional culture using a radial flow bioireactor induces matrix metalloprotease 7-mediated EMT-like process in tumor cells via TGF beta 1/Smad pathway

Shun-Ichi Shibata, Hideki Marushima, Tadashi Asakura, Tomokazu Matsuura, Homare Eda, Katsuhiko Aoki, Hiroshi Matsudaira, Kazu Ueda, Kiyoshi Ohkawa

INTERNATIONAL JOURNAL OF ONCOLOGY, 34(5) 1433-1448, May, 2009
The association of deamidation of Bcl-xL and translocation of Bax to the mitochondria through activation of JNK in the induction of apoptosis by treatment with GSH-conjugated DXR

Tadashi Asakura, Kazuhiro Maeda, Hiroko Omi, Hiroshi Matsudaira, Kiyoshi Ohkawa

INTERNATIONAL JOURNAL OF ONCOLOGY, 33(2) 389-395, Aug, 2008
Conformational change in the active center region of GST P1-1, due to binding of a synthetic conjugate of DXR with GSH, enhanced JNK-mediated apoptosis

Tadashi Asakura, Atsuko Sasagawa, Hitoshi Takeuchi, Shun-ichi Shibata, Hideki Marushima, Satoshi Mamori, Kiyoshi Ohkawa

APOPTOSIS, 12(7) 1269-1280, Jul, 2007
Relationship between expression of drug-resistance factors and drug sensitivity in normal human renal proximal tubular epithelial cells in comparison with renal cell carcinoma.

Oncology Report, 14, 601-7, 2005
Chemotherapeutic agent which induced mitochondrial apoptosis.

Asakura T, Ohkawa K

Current Cancer Drug Targets, 4 570-83, 2004
Establishment and some characteristics of epoximicin (a proteasome inhibitor) resistant variants of the human squamous cell cartinoma cell line, A431.

Int. J. Oncol., 24: 425-33., 2004
Chemotherapeutic agent which induced mitochondrial apoptosis.

Current Cancer Drug Targets, 4: 570-83., 2004
Acetaldehyde-induced growth retardation and micro-heterogeneity of the sugar chain in transferrin synthesized by Hep.G2 cells."jointly authored"

Alcoholism :Clin.Exp.Res., 26, 32S-7S, 2002
Relationship between cytocidal activity and glutathione-S-transferase inhibition using doxorubicin coupled to stereoisomers of glutathione with different substrate specificity

Yukio Hashizume, Tadashi Asakura, Tadao Oikawa, Takahiro Yamauchi, Kenji Soda, Kiyoshi Ohkawa

Anti-Cancer Drugs, 12(6) 549-554, 2001
Glutathione-S-transferase-π expression regulates sensitivity to glutathione-doxorubicin conjugate

Ken-Ichi Tashiro, Tadashi Asakura, Chieko Fujiwara, Kiyoshi Ohkawa, Yoshio Ishibashi

Anti-Cancer Drugs, 12(8) 707-712, 2001
Suppression of GST-P by treatment with glutathione-doxorubicin conjugate induces potent apoptosis in rat hepatoma cells

Asakura T, Hashizume Y, Tashiro K, Searashi Y, Ohkawa K, Nishihira J, Sakai M, Shibasaki T

Int.J.Cancer, 94(2) 171-177, 2001
Relationship between cytocidal activity and glutathione S-transferase inhibition using doxorubicin coupled to stereoisomers of glutathione which are different substrate specificity."jointly authored"

Anti-Cancer Drugs, 12, 549-54, 2001
Level of glutathione S-transferase-p expression regulates sensitivity to glutathione-doxorubicin conjugate."jointly authored"

Anti-Cancer Drugs, 12, 707-12, 2001
Suppression of GST-P by treatment with glutathione-doxorubicin conjugate induces potent apoptosis in rat hepatoma cells

Int.J.Cancer, 94, 171-7, 2001
Calpain inhibitor inhibits secretory granule maturation and secretion of GH."jointly authored"

Neuroreport, 11, 4007-11, 2000
Caspase-3 activation during apoptosis caused by doxorubicin-glutathione conjugate.

Asakura, T, Sawai, T, Hashidume, Y, Ohkawa, Y, Yokoyama, S, Ohkawa, K

British journal of cancer, 80(5/6) 711-715, 1999
Calpain inhibitor causes accumulation of ubiquitinated P-glycoprotein at the cell surface :possible role of calpain in P-glycoprotein turnover."jointly authored"

Int.J.Oncology, 15, 677-86, 1999
Caspase-3 activation during doxorubicin conjugated with glutathione-mediated apoptosis

Br. J. Cancer, 80(5/6) 711-715, 1999
Intracellular distribution of bovine serum albumin-conjugated doxorubicin and multidrug resistance"jointly authored"

Jpn. J. Cancer Chemother., 24(1) 87, 1997
Drug conjugate of doxorubicin with glutathione is a potent reverser of multidrug resistance in rat hepatoma cells

Anti-Cancer Drugs, 8(2) 199, 1997

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Presentations

30

Proteasome inhibitor, epoxomicin耐性細胞におけるE-cadherin発現消失を介した転移能の獲得.

第68回日本癌学会総会., 2009
Preparation of an ultrasound contrast of CD147 target immunomicrobubble.

第68回日本癌学会総会., 2009
解糖系阻害剤3-ブロモピルビン酸の抗癌作用に対するMCT1- CD147複合体の役割(Role of MCT1-CD147 complex in the anti-tumor effect of glycolytic inhibitor, 3-bromopyruvate).

第68回日本癌学会総会., 2009
ドキソルビシンおよびグルタチオン修飾ドキソルビシンによるによるアポトーシス誘導の解析

日本薬学会第129年会, 2009
Approach to imaging nanobubbles at high sensitivity (ナノバブル高感度イメージングの試み)

10th US Contrast（第10回国際造影超音波学会）, 2008
3-ブロモピルビン酸の抗癌作用に対するCD147-MCT複合体の役割 (Role of CD147-MCT complex in the anticancer effect of 3-bromopyruvate)

第81回日本生化学会大会, 2008
ラジアルフロー型バイオリアクタを用いた三次元培養下で細胞が示すEMTはTGF1/Smad, Wnt/LEF1が関与する (3-D culture using a Radial Flow Bioreactor induces EMT like process in tumor cells via TGF1/Smad and Wnt/LEF1 pathways.)

第67回日本癌学会総会, 2008
グルタチオン修飾ドキソルビシンによるアポトーシス誘導におけるプロヒビチンの関与 (Regulation of GSH-DXR conjugate-induced apoptosis by prohibitin in carcinoma cells.)

第67回日本癌学会総会, 2008
ラジアルフロー型バイオリアクタを用いた三次元培養下で細胞が示すEMTはTGFb1/Smad, Wnt/LEF1が関与する (3-D culture using a Radial Flow Bioreactor induces EMT like process in tumor cells via TGFb1/Smad and Wnt/LEF1 pathways.)

第67回日本癌学会総会, 2008
ドキソルビシンおよびグルタチオン修飾ドキソルビシンによるアポトーシスのシグナル伝達メカニズム.

日本薬学会第１２８年会., 2008
ドキソルビシンおよびグルタチオン修飾ドキソルビシンによるアポトーシス誘導メカニズム解析

日本薬学会関東支部大会, 2007
癌細胞膜表面高発現糖タンパク質CD147を標的とした化学療法の検討：Target chemotherapy of anti-CD147 antibody-liposome encapsulated GSH-DXR conjugate on CD147-expressed carcinoma cells.

第６６回日本癌学会総会, 2007
培養肝癌細胞における抗癌剤短時間暴露の有効性の検討： (To examine whether antineoplastic drugs would be effective to hepatocellular carcinoma (HCC) in a short exposure.).

第43回日本肝癌研究会., 2006
Deveropment of a novel method for purifying SDS-solubilized ubiquitin-protein conjugates.

20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress. 第79回日本生化学会大会.第29回日本分子生物学会大会., 2006
癌細胞膜表面高発現糖タンパク質CD147を標的とした化学療法の検討 (Target chemotherapy of anti-CD147 antibody-DXR conjugate on CD147 high expressed carcinoma cells.).

第65回日本癌学会総会., 2006
GSH結合DXRによるアポトーシス誘導はJNK活性化を介したBaxの細胞質からミトコンドリアへの移行が関与する (Translocation of Bax from cytoplasm to mitochondria through the activation of JNK induces apoptosis by treatment with GSH-conjugated DXR).

第64回日本癌学会総会, 2005
グルタチオン-ドキソルビシン複合体による脱アミド化Bcl-xLのミトコンドリアからの遊離がアポトーシスを誘導する (Bcl-xL-deamidation and liberation to the cytoplasma was enhanced apoptosis by treatment with GSH-DXR rather than DXR)

第77回日本生化学会大会, 2005
13C呼気試験のin vitro３次元培養系への応用.

第21回13C医学応用研究会・第8回日本呼気病態生化学研究会（合同学術集会）, 2005
GST P1-1はBcl-xL脱アミド化を介したアポトーシス誘導の抑制に関与する (Relation of GST P1-1 to suppression of Bcl-xL deamidation-induced apoptosis)

第63回日本癌学会総会, 2004
グルタチオン-ドキソルビシン複合体はBcl-xLの脱アミド化を介してアポトーシスを誘導する (Doxorubicin conjugated with glutathione induced apoptosis via Bcl-xL deamidation)

第77回日本生化学会大会, 2004

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Teaching Experience

3

Professional Memberships

4

Research Projects

2

癌の化学療法

基礎科学研究, 1995
Cancer chemotherapy

Basic Science Research Program, 1995