2014年4月
Distinct signaling pathways leading to the induction of human beta-defensin 2 by stimulating an electrolyticaly-generated acid functional water and double strand RNA in oral epithelial cells
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
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- 巻
- 34
- 号
- 2
- 開始ページ
- 97
- 終了ページ
- 103
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3109/10799893.2013.862272
- 出版者・発行元
- INFORMA HEALTHCARE
Defensins, a major family of cationic antimicrobial peptides, play important roles in innate immunity. In the present study, we investigated whether double-stranded RNA (dsRNA), a by-product of RNA virus replication, can induce human beta-defensins-2 (hBD-2) expression in oral epithelial cells (OECs). We also examined the hBD-2-inducible activity of acid-electrolyzed functional water (FW). The results indicated that both dsRNA-and FW-induced hBD-2 expression in OECs. The induction efficiency was much higher for FW than for dsRNA. FW-induced production of hBD-2 was clearly observed by immunofluorescence staining. A luciferase assay was performed with 1.2 kb of the 50-untranslated region (50-UTR) of the hBD-2 gene. The results indicated that the nuclear factor-kappa B (NF-kappa B)-binding site proximal to the translation initiation site was indispensable for dsRNA-stimulated hBD-2 expression, but not in the case of FW. Moreover, FW-stimulated hBD-2 expression did not depend on NF-kappa B activity; instead, FW inhibited NF-kappa B activity. Pretreatment of the cells with specific inhibitors against NF-kappa B further confirmed NF-kappa B-independent hBD-2 induction by FW. In analogy to the results for intestinal epithelial cells (IECs), the dsRNA signal, but not FW, was sensed by toll-like receptor 3 (TLR3) in OECs. These results suggested that hBD-2 expression induced by dsRNA and FW is regulated by distinct mechanisms in OECs.
- リンク情報
- ID情報
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- DOI : 10.3109/10799893.2013.862272
- ISSN : 1079-9893
- eISSN : 1532-4281
- Web of Science ID : WOS:000333099100005