The presence of yeasts in periodontal pockets is well known and Candida albicans (C. albicans) is thespecies most commonly isolated from theoral cavity. Theimmuneresponse and anti-candidal activity of oral epithelium cells play a key role in the host defense against C. albicans infection. Rat gingival tissue was infected with C. albicans ATCC90029. Total RNA was extracted from the epithelium and mRNA levels were monitored using DNA microarray. By ingenuity pathway analysis demonstrated that TNF and IL-1 stimulated gene expression of CC type chemokine CCL2, also known as monocyte chemoattractant protein-1 (MCP-1), through NF-κB pathway. Altered mRNA level of CCL2 by rat gingival tissue was infected with C. albicans was confirmed by reverse transcription polymerase chain reaction. The immunohistochemical examination for CCL2 production was carried out. As a result, stronger immunoreactivity against CCL2 was observed in the rat gingival epithelium infected with C. albicans.