2013年
Cloning and characterization of ribonuclease T2 gene (RNHe30) from the basidiomycete, Hericium erinaceum
Mycoscience
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- 巻
- 54
- 号
- 3
- 開始ページ
- 188
- 終了ページ
- 197
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.myc.2012.09.011
- 出版者・発行元
- Elsevier B.V.
A gene encoding a ribonuclease T2 (RNase T2) family enzyme, RNHe30, was cloned from Hericium erinaceum by PCR. The deduced amino acid sequence from the complimentary DNA (cDNA) (1074 bp) encodes a 302-aa protein (RNase He30) that has the consensus amino acid sequences of RNase T2 family enzymes including the putative signal peptide. The presence of five introns in the genomic DNA was confirmed by comparison of the cDNA and genomic DNA sequences. The promoter region contains a putative CAAT box and a consensus TATA box. Genes coding homologous enzymes were also identified in various other basidiomycetes. A phylogenetic tree of RNase T2s from these fungi was constructed from a multiple alignment of the deduced amino acid sequences. The tree showed that the enzymes were divided into two main groups. © 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.
- ID情報
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- DOI : 10.1016/j.myc.2012.09.011
- ISSN : 1618-2545
- ISSN : 1340-3540
- SCOPUS ID : 84875430493