論文

査読有り
2012年7月

Three-dimensional observation of the mouse embryo by micro-computed tomography: Meckel's cartilage, otocyst, and/or muscle of tongue

ODONTOLOGY
  • Hidekazu Aoyagi
  • ,
  • Shin-ichi Iwasaki
  • ,
  • Hideki Yoshizawa
  • ,
  • Kohzo Tsuchikawa

100
2
開始ページ
137
終了ページ
143
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10266-011-0041-2
出版者・発行元
SPRINGER

Three-dimensional observation during embryogenesis is possible with micro-computed tomography, but there are no observations of organ size. In this paper, three examples of three-dimensional observation of organs by micro-CT are tried. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained enbloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-computed tomography and image processing was performed. The entire Meckel's cartilage and its relation in the mandible, as well as the complex structure of the otocyst, are easily visualized. Although it is difficult to extract detailed structures of the tongue muscles, it is possible to identify the inner and external tongue muscles. Relation among the organs and other are easily visualized. Three-dimensional observation by micro-computed tomography is an important technology for visualization of embryogenesis and could be used in organ culture.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1007/s10266-011-0041-2
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306594200003&DestApp=WOS_CPL

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