論文

査読有り
2010年2月

Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion

ODONTOLOGY
  • Hidekazu Aoyagi
  • ,
  • Kohzo Tsuchikawa
  • ,
  • Shin-ichi Iwasaki

98
1
開始ページ
26
終了ページ
30
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10266-009-0112-9
出版者・発行元
SPRINGER

The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1007/s10266-009-0112-9
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274471100003&DestApp=WOS_CPL

エクスポート
BibTeX RIS