論文

査読有り
2015年2月

The perivascular phenotype and behaviors of dedifferentiated cells derived from human mature adipocytes

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Ning Song
  • ,
  • Liang Kou
  • ,
  • Xiao-Wen Lu
  • ,
  • Atsunori Sugawara
  • ,
  • Yutaka Shimizu
  • ,
  • Min-Ke Wu
  • ,
  • Li Du
  • ,
  • Hang Wang
  • ,
  • Soh Sato
  • ,
  • Jie-Fei Shen

457
3
開始ページ
479
終了ページ
484
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2015.01.033
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Derived from mature adipocytes, dedifferentiated fat (DFAT) cells represent a special group of multipotent cells. However, their phenotype and cellular nature remain unclear. Our study found that human DFAT cells adopted perivascular characteristics and behaviors. Flow cytometry and immunofluorescent staining revealed that human DFAT cells positively expressed markers highly related to perivascular cell lineages, such as CD140b, NG2 and desmin, but were negative for common endothelial markers, including CD31, CD34, and CD309. Furthermore, DFAT cells displayed vascular network formation ability in Matrigel, and they noticeably promoted and stabilized the vessel structures formed by human umbilical vascular endothelial cells (HUVECs) in vitro. These results provide novel evidence on the pericyte nature of human DFAT cells, further supporting the recent model for the perivascular origin of adult stem cells, in which tissue-specific progenitor cells in mesenchymal tissues associate with blood vessels, exhibiting perivascular characteristics and functions. (C) 2015 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2015.01.033
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351254700040&DestApp=WOS_CPL