論文

査読有り
2013年2月

Specificity of Antimicrobial Peptide LL-37 to Neutralize Periodontopathogenic Lipopolysaccharide Activity in Human Oral Fibroblasts

JOURNAL OF PERIODONTOLOGY
  • Wiroj Suphasiriroj
  • ,
  • Masato Mikami
  • ,
  • Hiromi Shimomura
  • ,
  • Soh Sato

84
2
開始ページ
256
終了ページ
264
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1902/jop.2012.110652
出版者・発行元
AMER ACAD PERIODONTOLOGY

Background: The antimicrobial peptide LL-37 is known to have a potent lipopolysaccharide (LPS)-neutralizing activity in various cell types. Because of observed heterogeneity within periodontopathogenic LPS, the authors hypothesized that LL-37 had specificity to neutralize such LPS activity. The present study, therefore, aims to investigate the LPS-neutralizing activity of LL-37 to various periodontopathogenic LPS in interleukin-8 (IL-8) production after challenging them in human oral fibroblasts.
Methods: Human periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (OF) were cultured from biopsies of periodontal ligament and gingival tissues. After cell confluence in 24-well plates, LPS (10 mu g/mL) from Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans were added with or without LL-37 (10 mu g/mL). After 18 hours, the supernatant was collected and analyzed in IL-8 production by enzyme-linked immunosorbent assay.
Results: All periodontopathogenic LPS statistically significantly induced IL-8 production in both PDLF and OF (P<0.01). After neutralization with LL-37, both PDLF and OF showed a statistically significant reduction in IL-8 production compared with LPS-treated groups without LL-37 (P<0.01), and the percentage of reduction in IL-8 production in PDLF appeared to be higher than in OF. In addition, the percentage of reduction in IL-8 production varied considerably according to each periodontopathogenic LPS.
Conclusions: The antimicrobial peptide LL-37 had an ability to suppress periodontopathogenic LPS-induced IL-8 production in both PDLF and OF. Its LPS-neutralizing activity revealed specificity to periodontopathogenic LPS and seemed to be dependent on the heterogeneity within LPS between different genera. J Periodontol 2013;84:256-264.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1902/jop.2012.110652
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000314791700016&DestApp=WOS_CPL