MISC

2004年11月

Tissue distribution and nucleotide sequence of bovine mRNA for salivary proline-rich protein P-B

ARCHIVES OF ORAL BIOLOGY
  • S Isemura
  • ,
  • S Watanabe
  • ,
  • S Fujiwara
  • ,
  • K Sanada

49
11
開始ページ
881
終了ページ
887
記述言語
英語
掲載種別
DOI
10.1016/j.archoralbio.2004.06.001
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

The tissue distribution of P-B was investigated to obtain information on the physiological significance of this proline-rich protein. To design primers and probes for a tissue distribution analysis, a polymerase chain reaction (PCR)-based cloning of bovine P-B cDNA was performed using tooth germ and the nucleotide sequence was determined. The cloned bovine P-B cDNA was composed of 356 bp and included the region corresponding to the mature P-B protein and part of the 3' non-coding sequence. This part of the sequence is identical to the corresponding region of human P-B cDNA from the submaxillary gland. DNA corresponding to the P-B mRNA was amplified by PCR using cDNAs from various bovine tissues including tooth germ, submaxillary gland, parotid gland, lachrymal gland, heart, liver, stomach, pancreas, spleen, kidney, adrenal, and ovary. A quantitative analysis indicated the heart, submaxillary gland, tooth germ and kidney to be major sites of P-B expression. The ubiquitous distribution of P-B mRNA among bovine tissues together with findings of the presence of genes hybridizable with a DNA probe for P-B among species such as human, bovine, rat, mouse, and yeast as reported previously suggested a fundamental physiological rote for this protein. (C) 2004 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.archoralbio.2004.06.001
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000224592600004&DestApp=WOS_CPL

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