論文

2021年

Immobilization of Target-Bound Aptamer on Field Effect Transistor Biosensor to Improve Sensitivity for Detection of Uncharged Cortisol

ELECTROCHEMISTRY
  • Hiroki Hayashi
  • ,
  • Ryo Toyama
  • ,
  • Ryota Takibuchi
  • ,
  • Sho Hideshima
  • ,
  • Shigeki Kuroiwa
  • ,
  • Naoto Kaneko
  • ,
  • Katsunori Horii
  • ,
  • Keishi Ohashi
  • ,
  • Toshiyuki Momma
  • ,
  • Tetsuya Osaka

89
2
開始ページ
134
終了ページ
137
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.5796/electrochemistry.20-00144
出版者・発行元
ELECTROCHEMICAL SOC JAPAN

Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors. (C) The Author(s) 2020. Published by ECSJ.

リンク情報
DOI
https://doi.org/10.5796/electrochemistry.20-00144
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000625867800012&DestApp=WOS_CPL
ID情報
  • DOI : 10.5796/electrochemistry.20-00144
  • ISSN : 1344-3542
  • eISSN : 2186-2451
  • Web of Science ID : WOS:000625867800012

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