論文

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2022年7月12日

Fabrication of a Deoxyribonuclease Sensor Based on the Electrical Characteristics of DNA Molecules

ACS Omega
  • Takahiro Himuro
  • ,
  • Shota Tsukamoto
  • ,
  • Yoji Saito

7
27
開始ページ
23549
終了ページ
23554
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acsomega.2c02069

In this study, we developed a sensing device that can detect deoxyribonuclease (DNase) based on the electrical properties of deoxyribonucleic acid (DNA). We estimated the equivalent circuit between the electrodes with immobilized DNA and investigated whether the characteristics of the electrodes change before and after the DNase reaction. This method detects DNase by simply evaluating the electrical properties of DNA without using a fluorescent reagent. Therefore, inexpensive and highly accurate measurements can be performed with simple operations. However, detection sensitivity must be increased for practical feasibility. Hence, we investigated whether DNA immobilization is restricted by changing the shape of the electrode to a triangle with sharp edges, which may improve the sensitivity of DNase. Additionally, we attempted to detect DNase from an extremely small amount of sample solution using a microchannel. The device was able to quantitatively analyze DNase I activity with a detection limit of 5.5 × 10-5unit/μL. The results demonstrate the effectiveness of the proposed sensing device for various medical applications.

リンク情報
DOI
https://doi.org/10.1021/acsomega.2c02069
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85135048010&origin=inward 本文へのリンクあり
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ID情報
  • DOI : 10.1021/acsomega.2c02069
  • eISSN : 2470-1343
  • SCOPUS ID : 85135048010

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