MISC

2008年9月

Raman optical activity of flagellar filaments of Salmonella: Unusually intense ROA from L-type self-assembled protein filaments and their possible higher level chiral organization

VIBRATIONAL SPECTROSCOPY
  • Tomonori Uchiyama
  • ,
  • Masashi Sonoyama
  • ,
  • Yoshiaki Hamada
  • ,
  • Rina K. Dukor
  • ,
  • Laurence A. Nafie
  • ,
  • Fumio Hayashi
  • ,
  • Kenji Oosawa

48
1
開始ページ
65
終了ページ
68
記述言語
英語
掲載種別
DOI
10.1016/j.vibspec.2007.12.008
出版者・発行元
ELSEVIER SCIENCE BV

The flagellar filament of Salmonella is an assembly of a single protein, flagellin. There are different helical and straight shape filaments from wild type and mutants. Raman and Raman optical activity (ROA) spectroscopies were employed to characterize the structure of flagellar filaments. The Raman spectra of normal and two straight filaments were almost same, but in the ROA spectra, intensive ROA peaks at amide I, amide III and skeletal vibrational regions were observed from only one of two straight filaments, L-type straight filaments. When the filaments were depolymerized or fragmented, the ROA bands disappeared on the scale of the large ROA intensities. It is thought that the ROA bands of L-type flagellar filaments are related to their shape, length and possible higher level chiral organization. (C) 2007 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1016/j.vibspec.2007.12.008
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000259283100012&DestApp=WOS_CPL