MISC

2006年11月

Raman spectroscopic study on the L-type straight flagellar filament of Salmonella

VIBRATIONAL SPECTROSCOPY
  • Tomonori Uchiyama
  • ,
  • Masashi Sonoyama
  • ,
  • Yoshiaki Hamada
  • ,
  • Mamoru Komatsu
  • ,
  • Rina K. Dukor
  • ,
  • Laurence A. Nafie
  • ,
  • Kenji Oosawa

42
2
開始ページ
192
終了ページ
194
記述言語
英語
掲載種別
DOI
10.1016/j.vibspec.2006.02.008
出版者・発行元
ELSEVIER SCIENCE BV

Structural characterization of the L-type straight flagellar filament of Salmonella was attempted with the use of Raman spectroscopy as the groundwork for addressing the structural basis responsible for molecular assembly and polymorphic transformation of the flagella filament that is a supramolecular assembly of a single protein molecule, flagellin. When the filament was depolymerized to flagellin monomer by heating, the amide I band showed remarkable upshift from 1653 to 1662 cm(-1) with broadening, whereas the frequency of the amide III band kept unchanged at similar to 1248 cm(-1). According to the experience rules on Raman frequency-structure relation, the spectral properties in the amide I and III regions are not necessarily in agreement with the structural features suggested by other analytical techniques on the structure. Further investigations on the vibrational spectra of the flagellar filament would be very helpful for re-examining the relationships between protein secondary structures and Raman frequency in the amide I and III regions. (C) 2006 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.vibspec.2006.02.008
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242764300004&DestApp=WOS_CPL