Aug, 2016
Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography
JOURNAL OF VETERINARY MEDICAL SCIENCE
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- Volume
- 78
- Number
- 8
- First page
- 1277
- Last page
- 1281
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1292/jvms.16-0052
- Publisher
- JAPAN SOC VET SCI
Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.
- Link information
- ID information
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- DOI : 10.1292/jvms.16-0052
- ISSN : 0916-7250
- eISSN : 1347-7439
- Pubmed ID : 27170431
- Web of Science ID : WOS:000382421600008