論文

査読有り
2014年1月

Discrimination of Mycobacterium abscessus subsp massiliense from Mycobacterium abscessus subsp abscessus in Clinical Isolates by Multiplex PCR

JOURNAL OF CLINICAL MICROBIOLOGY
  • Kazue Nakanaga
  • ,
  • Tsuyoshi Sekizuka
  • ,
  • Hanako Fukano
  • ,
  • Yumi Sakakibara
  • ,
  • Fumihiko Takeuchi
  • ,
  • Shinpei Wada
  • ,
  • Norihisa Ishii
  • ,
  • Masahiko Makino
  • ,
  • Makoto Kuroda
  • ,
  • Yoshihiko Hoshino

52
1
開始ページ
251
終了ページ
259
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/JCM.01327-13
出版者・発行元
AMER SOC MICROBIOLOGY

The rapidly growing mycobacterium M. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases and of infections following cosmetic surgery and postsurgical procedures. M. abscessus sensu lato can be divided into at least three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. Clinical isolates of rapidly growing mycobacteria were previously identified as M. abscessus by DNA-DNA hybridization. More than 30% of these 117 clinical isolates were differentiated as M. abscessus subsp. massiliense using combinations of multilocus genotyping analyses. A much more cost-effective technique to distinguish M. abscessus subsp. massiliense from M. abscessus subsp. abscessus, a multiplex PCR assay, was developed using the whole-genome sequence of M. abscessus subsp. massiliense JCM15300 as a reference. Several primer sets were designed for single PCR to discriminate between the strains based on amplicons of different sizes. Two of these single-PCR target sites were chosen for development of the multiplex PCR assay. Multiplex PCR was successful in distinguishing clinical isolates of M. abscessus subsp. massiliense from samples previously identified as M. abscessus. This approach, which spans whole-genome sequencing and clinical diagnosis, will facilitate the acquisition of more-precise information about bacterial genomes, aid in the choice of more relevant therapies, and promote the advancement of novel discrimination and differential diagnostic assays.

Web of Science ® 被引用回数 : 36

リンク情報
DOI
https://doi.org/10.1128/JCM.01327-13
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000329222400036&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/JCM.01327-13
  • ISSN : 0095-1137
  • eISSN : 1098-660X
  • Web of Science ID : WOS:000329222400036

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