This experimental study was performed on rats to investigate a suitable concentration level of sodium fluoride on bone metabolism. The experimental osteoporotic rats were divided into two groups; a sodium fluoride group and a non-sodium fluoride group. The sodium fluoride group was divided into three more groups according to the concentration level of sodium fluoride, Ippm, 5ppm, and 10ppm. Then, the rats of these four groups were respectively divided into a high calcium diet group and a control diet group. Microdensitometer was used to measure, dry weight and ash weight in the femur. The rate of trabecular bone areas was measured in the proximal epiphysis of the tibia. Histomorphometrial parameters were measured in the third and fourth coccyx. Results: Compared to the non-sodium fluoride group, the sodium fluoride group showed increase of trabecular bone in microdensitometer measurements, dry weight, and ash weight in the femur. The rate of trabeculor bone in the proximal epiphysis of the tibia and the value of the histamorphometry in the coccyx indicated the same result. Especially 1 ppm of sodium fluoride proved to be the most efficient value for increase of trabecular bone. This study concluded that around 1 ppm of sodium fluoride was the most suitable concentration level. This study also proved that a combined therapy of 1 ppm of sodium fluoride and calcium increased the volume of cortical and trabecular bone.