MISC

2001年8月

Neurotrophin secretion from cultured microglia

JOURNAL OF NEUROSCIENCE RESEARCH
  • K Nakajima
  • ,
  • S Honda
  • ,
  • Y Tohyama
  • ,
  • Y Imai
  • ,
  • S Kohsaka
  • ,
  • T Kurihara

65
4
開始ページ
322
終了ページ
331
記述言語
英語
掲載種別
DOI
10.1002/jnr.1157
出版者・発行元
WILEY-LISS

Because microglia have been suggested to produce neurotrophins, we tested this ability in vitro. Rat primary microglia were found to constitutively secrete a limited amount of brain-derived neurotrophic factor (BDNF), but nerve growth factor (NGF) and neurotrophin-3 (NT-3) were undetectable in the conditioned medium. Stimulation of the cells with lipopolysaccharide (LPS) increased BDNF secretion, and induced NGF secretion. As a first step to examine this regulation system, the association of protein kinase C (PKC) was pharmacologically analyzed. A PKC activator, phorbol-12-myristate-13-acetate, enhanced the secretion of BDNF. Pre-treatment of microglia with a PKC inhibitor, bisindolylmaleimide, suppressed LPS-stimulated BDNF secretion as well as the constitutive one. These results suggest that the PKC signaling cascade is closely associated with BDNF secretion. Among PKC isoforms, PKC alpha probably plays a role in BDNF secretion, based on the results of experiments using a specific PKC activator, 1-oleoyl-2-acetyl-sn-glycerol, and a specific PKC inhibitor, Go 6976, and by immunoblotting. Taken together, these findings suggest that the secretion of BDNF from microglia is regulated through PKC alpha -associated signal transduction mechanism. (C) 2001 Wiley-Liss, Inc.

Web of Science ® 被引用回数 : 162

リンク情報
DOI
https://doi.org/10.1002/jnr.1157
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000170237500007&DestApp=WOS_CPL

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