2006年9月
Systemic labeling and visualization of dental sensory nerves by the novel fluorescent marker AM1-43
ANATOMICAL SCIENCE INTERNATIONAL
- 巻
- 81
- 号
- 3
- 開始ページ
- 181
- 終了ページ
- 186
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1447-073x.2006.00147.x
- 出版者・発行元
- BLACKWELL PUBLISHING
Systemic labeling of sensory nerves was performed by injecting a small amount of the styryl dye AM1-43 subcutaneously to the back skin of 4-week-old mice in order to determine its ability to stain sensory nerves. One or 3 days later, dental tissues were fixed and cryosectioned. Molars showed bright nerve fibers in the periodontal ligament and pulp. Nervefibers in dentinal tubules approximately 100 mu m from the pulp were also labeled. In the incisor, there were only few labelings in the pulp, although free nerve endings and Ruffini-type mechanosensors in the periodontal ligament on the lingual side were brightly labeled. The AM1-43-positive fibers were also labeled by anti-PGP9.5. AM1-43 is an excellent marker for sensory nerves and it may be useful for further investigations of dental innervation and in exploring new analgesics for tooth pain.
- リンク情報
- ID情報
-
- DOI : 10.1111/j.1447-073x.2006.00147.x
- ISSN : 1447-6959
- PubMed ID : 16955669
- Web of Science ID : WOS:000240198600005