MISC

1995年4月

STRUCTURAL STUDY ON THE FREE LIPID-A ISOLATED FROM LIPOPOLYSACCHARIDE OF PORPHYROMONAS-GINGIVALIS

JOURNAL OF BACTERIOLOGY
  • H KUMADA
  • ,
  • Y HAISHIMA
  • ,
  • T UMEMOTO
  • ,
  • KI TANAMOTO

177
8
開始ページ
2098
終了ページ
2106
記述言語
英語
掲載種別
出版者・発行元
AMER SOC MICROBIOLOGY

The chemical structure of lipid A isolated from Porphyromonas gingivalis lipopolysaccharide was elucidated by compositional analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy. The hydrophilic backbone of free lipid A was found to consisted of beta(1,6)-linked D-glucosamine disaccharide 1-phosphate. (R)-3-Hydroxy-15-methylhexadecanoic acid and (R)-3-hydroxyhexadecanoic acid are attached at positions 2 and 3 of the reducing terminal residue, respectively, and positions 2' and 3' of the nonreducing terminal unit are acylated with (R)-3-O-(hexadecanoyl)-15-methylhexadecanoic acid and (R)-3-hydroxy-13-methyltetradecanoic acid, respectively. The hydroxyl group at position 4' is partially replaced by another phosphate group, and the hydroxyl groups at positions 4 and 6' are unsubstituted. Considerable heterogeneity in the fatty acid chain length and the degree of acylation and phosphorylation was detected by liquid secondary ion-mass spectrometry (LSI-MS). A significant pseudomolecular ion of lipid A at m/z 1,769.6 [M-H](-) corresponding to a diphosphorylated GlcN backbone bearing five acyl groups described above was detected in the negative mode of LSI-MS. Predominant ions, however, were observed at mit 1,433.9 [M-H](-) and m/z 1,349.0 [M-H](-), each representing monophosphoryl lipid A lacking (R)-3-hydroxyhexadecanoic and (R)-3-hydroxy-13-methyltetradecanoic acids, respectively. The presence of mono- and diphosphorylated lipid A species was also confirmed by LSI-MS of de-O-acylated lipid A (mit m/z 955.3 and 1,035.2, respectively).

Web of Science ® 被引用回数 : 138

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995QR55600024&DestApp=WOS_CPL

エクスポート
BibTeX RIS