MISC

1995年9月

INFLAMMATORY CYTOKINE GENE-EXPRESSION IN HUMAN PERIODONTAL-LIGAMENT FIBROBLASTS STIMULATED WITH BACTERIAL LIPOPOLYSACCHARIDES

INFECTION AND IMMUNITY
  • Y YAMAJI
  • ,
  • T KUBOTA
  • ,
  • K SASAGURI
  • ,
  • S SATO
  • ,
  • Y SUZUKI
  • ,
  • H KUMADA
  • ,
  • T UMEMOTO

63
9
開始ページ
3576
終了ページ
3581
記述言語
英語
掲載種別
出版者・発行元
AMER SOC MICROBIOLOGY

The effects of Porphyromonas gingivalis lipopolysaccharide (P-LPS) and Escherichia coli lipopolysaccharide (E-LPS) on the gene expression and production of inflammatory cytokines of human periodontal ligament fibroblasts (HPLF) were examined by a Northern (RNA blot) assay and enzyme-linked immunosorbent assay, respectively. mRNAs for interleukin-6 (IL-6), IL-8, and transforming growth factor beta (TGP-P) were detected in HPLF cells, but IL-1 alpha, IL-1 beta, tumor necrosis factor alpha, transforming growth factor alpha, and granulocyte-macrophage colony-stimulating factor were not detected by reverse transcription-PCR. The expression of TGF-P mRNA was not influenced by either LPS, P-LPS (1 to 10 mu g/ml) and E-LPS (100 mu g/ml) markedly stimulated the expression of IL-6 and IL-8 mRNAs compared with the control. The synthesis of IL-6 and IL-8 was also stimulated by 10 and 100 mu g of both LPSs per mi, but IL-8 synthesis was not stimulated with E-LPS at 1 mu g/ml. Secretion of IL-6 and IL-8 into the culture medium was detected at 6 and 3 h, respectively, after exposure to P-LPS (10 mu g/ml). These findings suggested that P. gingivalis leads to periodontal tissue destruction and alveolar bone resorption through IL-6 and IL-8 released from HPLF cells stimulated with its LPS.

Web of Science ® 被引用回数 : 95

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Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995RQ79200051&DestApp=WOS_CPL

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