MISC

1993年

Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7

Bioscience, Biotechnology, and Biochemistry
  • Tsujibo Hiroshi
  • ,
  • Orikoshi Hideyuki
  • ,
  • Imada Chiaki
  • ,
  • Okami Yoshiro
  • ,
  • Miyamoto Katsushiro
  • ,
  • Inamori Yoshihiko

57
8
開始ページ
1396
終了ページ
1397
記述言語
英語
掲載種別
DOI
10.1271/bbb.57.1396
出版者・発行元
社団法人日本農芸化学会

Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of β-glycosidic linkage of chitin.

リンク情報
DOI
https://doi.org/10.1271/bbb.57.1396
CiNii Articles
http://ci.nii.ac.jp/naid/110002676518
CiNii Books
http://ci.nii.ac.jp/ncid/AA10824164
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/7764022
URL
https://jlc.jst.go.jp/DN/JALC/00079819282?from=CiNii
ID情報
  • DOI : 10.1271/bbb.57.1396
  • ISSN : 0916-8451
  • ISSN : 1347-6947
  • CiNii Articles ID : 110002676518
  • CiNii Books ID : AA10824164
  • PubMed ID : 7764022

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