論文

査読有り
2014年10月

Identification and characterization of Aeromonas hydrophila genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Tatsuya Funahashi
  • ,
  • Tomotaka Tanabe
  • ,
  • Jun Maki
  • ,
  • Katsushiro Miyamoto
  • ,
  • Hiroshi Tsujibo
  • ,
  • Shigeo Yamamoto

78
10
開始ページ
1777
終了ページ
1787
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2014.932669
出版者・発行元
TAYLOR & FRANCIS LTD

We found that, under iron-limiting conditions, Aeromonas hydrophila ATCC 7966(T) could utilize the xenosiderophore desferrioxamine B (DFOB) for growth by inducing the expression of its own outer membrane receptor. Two consecutive genes, desR and desA, were selected as candidates involved in DFOB utilization. The presence of the ferric-uptake regulator boxes in their promoters suggested that these genes are under iron-dependent regulation. Mutation of desA, a gene that encodes the outer membrane receptor of ferrioxamine B, disrupted the growth of the amonabactin-deficient mutant in the presence of DFOB. beta-Galactosidase reporter assays and reverse transcriptase-quantitative PCR demonstrated that desR, a gene that encodes an AraC-like regulator homolog is required for the induction of desA transcription in the presence of DFOB and under iron-limiting conditions. The functions of desA and desR were analyzed using complementation experiments. Our data provided evidence that DesA is powered primarily by the TonB2 system.

リンク情報
DOI
https://doi.org/10.1080/09168451.2014.932669
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25273145
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000342843300024&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2014.932669
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • PubMed ID : 25273145
  • Web of Science ID : WOS:000342843300024

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